The major propose of this research was to investigate the anatomical differences between male and female from Thai cadavers by using hyoid bone since it is hardly broken and destroyed among unstable condition. Furthermore, the data of this result may help the investigative and judicial system. Sixty one hyoid bones had been used; 38 from male and 23 from female at the age ranging from 21-99 years old. Seventeen measurements were determined on each bone. Discriminant analysis was required to transform the variables measurements. P-value that less than 0.05 was considered as significant differences. Left greater cornu revealed significant differences (p-value = 0.00) at M5 and M6 points. And part of right greater cornu revealed statistical significant differences (p-value = 0.00) at M2 and M11 points. The most accuracy percent values of male and female is at M4 (83.6%). And the result from chi-square analysis between sex and osseous fusion showed no significant differences. Thus, sex and osseous fusion in Thai populations has no correlation.